Sequencing the Rice Genome

Efficiency to Discovery Transgenic Loci in GM Rice Using Next Generation Sequencing Whole Genome Re-sequencing

Molecular characterization technology in genetically modified organisms, in addition to how transgenic biotechnologies are developed now require full transparency to assess the risk to living modified and non-modified organisms. Next generation sequencing (NGS) methodology is suggested as an effective means in genome characterization and detection of transgenic insertion locations. In the prese...

Sequencing the Bonobo Genome

The Bonobo Genome Consortium generated DNA sequencing reads representing the genome of a single bonobo individual. The data consisted of almost 270 million fragment sequences generated on FLX machines from 454 Life Sciences. The fragments derived from FLX standard and Titanium chemistries, and from paired and unpaired protocols. The data was assembled at the J. Craig Venter Institute with the o...

Sequencing the Chimpanzee Genome

Sequence of the chimpanzee (Pan troglodytes) genome should be a high priority. The chimpanzee genome sequence will have a major impact on our understanding of human disease, human evolution, and human population genetics. We propose that the first phase of a project to obtain chimpanzee sequence consist of 3-4X whole-genome shotgun coverage, with the sequence obtained from multiple individuals ...

Sequencing the maize genome.

Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x cove...

Sequencing the Human Genome